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. 2018 Oct 23;9:2442. doi: 10.3389/fimmu.2018.02442

Figure 2.

Figure 2

Anti-TNP IgG1 antibodies with low fucose low galactose (–F–G) and low fucose high bisection (–F+B) inhibit phagocytosis of anti-RhD-opsonized erythrocytes significantly better than unmodified IgG1 antibodies in both M-CSF and GM-CSF cultured macrophages. (A) Anti-TNP antibodies with different Fc-glycovariants (indicated on the x-axis) were used, at 1 μg/mL, to inhibit the phagocytosis of polyclonal anti-RhD opsonized erythrocytes by monocyte-derived macrophages cultured with GM-CSF (left) or M-CSF (right). Data were normalized to the percentage of macrophages that phagocytosed anti-RhD opsonized erythrocytes when inhibited with 1 μg/mL unmodified anti-TNP IgG1 (which resulted in about 30% phagocytosis compared to the unblocked control). Data represent mean and standard error of the mean (n = 3 batches, always tested in at least 4 times per batch). Glycovariants are compared to unmodified IgG, analysis is performed by multiple t-tests, corrected for multiple comparisons using Holm-Sidak, *p < 0.01; **p < 0.001; ***p < 0.0001. (B) Schematic representation of the bi-antennary Fc glycan of the two best blocking glycovariants.