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. 2018 Oct 29;8:15942. doi: 10.1038/s41598-018-34182-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transverse sections of distal intestine photomicrograph of the juvenile barramundi (Panel A–D). All sections are stained with H&E. Scale bar, 50 μm, inset 20 µm. X-axis represents the TH inclusion levels of 0 (control), 5, 10, 15 and 20 are considered as experimental treatments (E–H). The distal intestine of juvenile barramundi is influenced by the inclusion of tuna hydrolysate (TH) in diets at varying levels for 8 weeks. The different measurements include GC = Goblet cells (Panel E), hF = fold height (Panel F), hMV = microvillous height (Panel G), ECS = external circumference of serosa (Panel H). Arrow point and cartoon with bracket both indicate hMV (Panel C). Data were represented as mean ± S.E., n = 5. Post ANOVA Tukey multiple comparison test was applied to compare the mean value of each treatment with the mean value of the control. Mean values significantly different from the control are noted with P < 0.05, P < 0.01 and P < 0.001.