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. 2018 Oct 29;8:15942. doi: 10.1038/s41598-018-34182-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serum lysozyme (A) and complement (B) activities of juvenile barramundi fed TH diets at different inclusion levels for 8 weeks. Data were expressed as mean ± SE. X-axis represents the TH inclusion levels of 0 (control), 5, 10, 15 and 20 are considered as experimental treatments. Different lowercase letters (a,b,c) denote statistically significant differences among pre-challenge, post-challenge-24 h and post- challenge- 7 d in the same treatment. Bars holding same uppercase letter (A) among the different experimental treatments are not statistically significant. (Multifactorial ANOVA; Tukey post-hoc test; not significant P > 0.05; significant P < 0.05; P < 0.001).