Table 3.
Formulation and proximate composition of the experimental diets for juvenile barramundi.
| Ingredients (g kg−1)a | Experimental diets | ||||
|---|---|---|---|---|---|
| Control | TH05 | TH10 | TH15 | TH20 | |
| Fish meal | 610.0 | 579.5 | 549.0 | 518.5 | 488.0 |
| Tuna hydrolysate | — | 30.5 | 61.0 | 91.5 | 122.0 |
| Wheat | 266.0 | 260.0 | 254.0 | 248.0 | 240.0 |
| Wheat starch | 20.0 | 20.0 | 20.0 | 20.0 | 20.0 |
| Fish Oil | 30.0 | 30.0 | 30.0 | 30.0 | 30.0 |
| Calcium carbonate | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Salt (NaCl) | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Vitamin premixb | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Casein | 63.0 | 69.0 | 75.0 | 81.0 | 89.0 |
| Cellulose | 6.0 | 6.0 | 6.0 | 6.0 | 6.0 |
| Proximate composition (% dry matter) | |||||
| Dry matter | 92.72 | 91.05 | 90.38 | 89.71 | 89.04 |
| Crude protein | 47.10 | 47.16 | 47.14 | 47.12 | 47.18 |
| Crude lipid | 9.99 | 9.88 | 9.96 | 9.94 | 9.92 |
| Ash | 13.04 | 12.56 | 12.09 | 11.61 | 11.14 |
| NFEc | 22.59 | 21.45 | 21.19 | 21.04 | 20.80 |
| Gross energy (MJkg−1) | 19.98 | 19.97 | 19.96 | 19.95 | 19.97 |
aSupplied by Specialty Feeds, Perth, Australia.
bVitamin premix (g/kg): iron, 10; copper, 1.5; iodine, 0.15;manganese, 9.5; zinc, 25; vitamin A retinol, 100 IU; vitamin D3, 100 IU; vitamin E, 6.25; vitamin K, 1.6; vitamin B1, 1; vitamin B2, 2.5; niacin, 20; vitamin B6, 1.5; calcium, 5.5; biotin, 0.1; folic acid, 0.4; inositol, 60; vitamin B12, 0.002; choline, 150; ethoxyquin, 0.125.
cNitrogen free extracts (NFE) = dry matter − (crude lipid + crude ash + crude protein).