Fig. 4. DEPTOR is a negative regulator of decidualization.

a, b Human endometrial stromal (hES) cells were infected with lentiviruses expressing two different DEPTOR shRNAs or scrambled shRNA and were then selected by puromycin for 5 days. a Cells were lysed and then analyzed by western blotting. b After 2 days of differentiation, the cells were lysed and subjected to quantitative RT-PCR. c Cells were transfected with Flag-DEPTOR, lysed, and analyzed by western blotting. d Cells were treated as described in (c), differentiated for 2 days, and then subjected to quantitative RT-PCR. Human GAPDH was used to normalize gene expression. All data are shown as the mean ± SD or are blots representative of three to five independent experiments. Student’s t-tests were performed to compare the indicated pairs of data. **P < 0.01