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. 2018 Oct 29;8:15915. doi: 10.1038/s41598-018-34315-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of Asparagine Synthetase (Asns) as a novel BA responsive gene. (A) Genes displaying >3-fold increase in expression with BA treatment at day 3 (relative to control) measured by RNA-sequencing. Genes ranked by significance (p < 0.05). (B) qPCR validation of Asns expression in all samples (Control, BA and GH; total n = 164). *p < 0.001 for time x treatment interaction (see Supplementary Table 2 for post hoc Bonferroni test comparisons). (C) Representative image of western blot for ASNS in control, BA and GH treated LD muscles at day 7 (see supplementary Figure 1 for full blot and gel). (D) Quantitation of ASNS protein abundance at day 7 from western blot. ^#p < 0.05 (ANOVA).