Table 2.

Hierarchical analysis of molecular variance (AMOVA) of COI, 16S, and 28S for all sampled lineages of Pygmarrhopalites and Pogonognathellus grouped by cave

Source of variation	COI					16S					28S
	df	SS	VC	V%	ϕ‐Statistics	df	SS	VC	V%	ϕ‐Statistics	df	SS	VC	V%	ϕ‐Statistics
Pygmarrhopalites
Among caves	15	1,785.70	20.53	28.62	ϕct = 0.29**	14	658.50	6.60	22.48	ϕct = 0.23*	15	346.28	1.80	10.70	ϕct = 0.11
Among samples within caves	13	829.91	34.20	47.68	ϕsc = 0.67**	12	343.73	16.46	56.08	ϕsc = 0.72**	13	238.20	10.00	60.00	ϕsc = 0.67**
Within samples	13	221.08	17.01	23.71	ϕst = 0.76***	12	75.50	6.29	21.44	ϕst = 0.79***	11	53.80	4.90	29.30	ϕst = 0.71***
Pogonognathellus
Among caves	10	337.90	11.61	56.37	ϕct = 0.56*	9	193.08	7.66	60.61	ϕct = 0.61*	9	11.27	0.03	2.50	ϕct = 0.03
Among samples within caves	13	130.02	8.49	41.21	ϕsc = 0.94	12	67.50	4.98	39.39	ϕsc = 1.00	11	13.50	0.68	56.22	ϕsc = 0.58
Within samples	2	1.00	0.50	2.43	ϕst = 0.98*	2	0.00	0.00	0.00	ϕst = 1.00*	1	0.50	0.50	41.28	ϕst = 0.03

df: degrees of freedom; SS: sum of squares; VC: variance components; V%: percent of variation.

Significance is based on 50,000 permutations: *p < 0.05, **p < 0.01, ***p < 0.001.