Table 4.

Hierarchical analysis of molecular variance (AMOVA) of COI and 16S for Pygmarrhopalites and Pogonognathellus focal OTUs grouped by (a) cave, (b) sinkhole area, and (c) by region relative to the Mississippi River

Source of variation	COI					16S
	df	SS	VC	V%	ϕ‐Statistics	df	SS	VC	V%	ϕ‐Statistics
(a) Cave
Pygmarrhopalites A10
Among caves	4	170.58	15.68	87.98	ϕct = 0.88	4	31.25	2.93	90.72	ϕct = 0.91
Among pops within caves	2	5.25	1.93	10.85	ϕsc = 0.9	2	0.75	0.3	9.28	ϕsc = 1
Within populations	6	1.25	0.21	1.17	ϕst = 0.99***	6	0	0	0	ϕst = 1***
Pogonognathellus T4
Among caves	8	104.05	5.87	92.47	ϕct = 0.93***	7	40	2.49	97.91	ϕct = 0.98***
Among pops within caves	11	5.22	−0.02	−0.35	ϕsc = −0.05	11	0.67	0.53	2.09	ϕsc = 1
Within populations	2	1	0.5	7.88	ϕst = 0.92*	2	0	0	0	ϕst = 1*
(b) Sinkhole area
Pygmarrhopalites A10
Among sinkhole areas	3	170.16	19.08	94.16	ϕct = 0.95*	3	31.25	3.54	96.06	ϕct = 0.96**
Among pops within areas	3	5.67	0.98	4.82	ϕsc = 0.82	3	0.75	0.15	3.94	ϕsc = 1
Within populations	6	1.25	0.21	1.03	ϕst = 0.99***	6	0	0	0	ϕst = 1***
Pogonognathellus T4
Among sinkhole areas	2	36.67	3	43.33	ϕct = 0.43***	2	16.27	1.42	50.83	ϕct = 0.51***
Among pops within areas	17	72.6	3.42	49.44	ϕsc = 0.87	16	24.4	1.38	49.17	ϕsc = 1
Within populations	2	1	0.5	7.23	ϕst = 0.93*	2	0	0	0	ϕst = 1
(c) Region
Pygmarrhopalites A10
Among regions	1	99.38	13.59	58.36	ϕct = 0.58*	1	21.92	3.38	72.7	ϕct = 0.73*
Among pops within regions	5	76.44	9.49	40.75	ϕsc = 0.98**	5	10.83	1.27	27.3	ϕsc = 1*
Within populations	6	1.25	0.2	0.89	ϕst = 0.99***	6	0	0	0	ϕst = 1***
Pogonognathellus T4
Among regions	1	7.29	0.38	6.77	ϕct = 0.07	1	2.83	0.15	6.79	ϕct =0.07
Among pops within regions	18	101.98	4.7	84.26	ϕsc = 0.9	17	37.83	2.01	93.21	ϕsc = 1
Within populations	2	1	0.5	8.97	ϕst = 0.91*	2	0	0	0	ϕst = 1

df: degrees of freedom; SS: sum of squares; VC: variance components; V%: percent of variation.

Significance is based on 50,000 permutations: *p < 0.05, **p < 0.01, ***p < 0.001.