Figure 2.

Cytokine capture assay for IFN-γ and TNF-α in CD8 T cells. Examples of gating strategies for sorting cells that were either IFN-γ and TNF-α positive. Cells were labeled with CD8 and (A) IFN-γ using a bispecific CD45/IFN-γ capture reagent; (B) TNF-α using a bispecific CD45/TNF-α capture reagent; or (C) TNF-α using a TNF-α capture assay with TNF-α processing inhibitor (TAPI-0). Stained cells were acquired and sorted on BD-FACS-Aria sorter.