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. 2018 Oct 23;9:2462. doi: 10.3389/fimmu.2018.02462

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Copyright © 2018 Saxena, Dagur, Desai and McCoy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFN-γ protein quantification at different mean fluorescent intensities (MFI). (A) Flow cytometry dot plot showing gating strategy for sorting CD8+IFN-γ+ cells at different MFI's using bin gating. The gates were set as such that the MFI values fall in a range of 100,000 with 8 different intensities following a two-fold incremental order and covering from the brightest to the dimmest IFN-γ expression on CD8 cells (B) Measured amounts of IFN-γ from cells sorted from various levels of staining for cells surface IFN-γ expressed as mean ± SD fluorescence intensity channel numbers (MFI).