FIGURE 5.

Overexpression of Kcnmb2 in CA1 region of dorsal hippocampus rescued LTP deficits in MD F1 mice. (A) LTP induced with a 100 Hz, 1 s tetanus in four groups of mice. Inserts, sample fEPSP traces recorded at 10 min post-tetanus. Three-way ANOVA with the between-subjects factors paternal diet (MD vs. CD) and AAV treatment (AAV-Kcnmb2 vs. AAV-control), and the within-subjects factor time. n = 11 slices from six MD-control mice, n = 23 slices from eight CD-control mice; n = 13 slices from six MD-Kcnmb2 mice and n = 12 slices from seven CD-Kcnmb2 mice. (B) Comparisons of early LTP (left, 0–10 min post-tetanus) and remaining LTP (right, 50–60 min post-tetanus) in SC-CA1 synapses pathway between CD and MD F1 neurons infected by control or Kcnmb2 virus. Two-way ANOVA followed by Tukey’s multiple comparisons test, n = 11 slices from six MD-control mice, n = 23 slices from eight CD-control mice; n = 13 slices from six MD-Kcnmb2 mice and n = 12 slices from seven CD-Kcnmb2 mice. The input–output curve (C) and paired-pulse ratio (PPR) (D) indicating no significant differences in basal synaptic transmission of SC-CA1 pathway between MD and CD F1 mice receiving control or Kcnmb2 virus injection. Three-way ANOVA, n = 23 slices from six MD-control mice, n = 35 slices from eight CD-control mice, n = 25 slices from six MD-Kcnmb2 mice and n = 28 slices from seven CD-Kcnmb2 mice. Inserts in (C), sample fEPSPs evoked by 40 μA (100 μs) stimulation delivered to SC-CA1 pathway. Inserts in (D), sample fEPSPs evoked by paired-pulse stimulation (40 μA, 100 μs) with ISI of 50 ms. ^∗∗P < 0.01 means significant difference. All data are shown as means ± SEM.