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. 2018 Oct 23;11:385. doi: 10.3389/fnmol.2018.00385

FIGURE 7.

FIGURE 7

Effect of transgenes on expression of neighboring endogenous genes. Expression of Slm1, Fat4, and Cdh6 mRNA from TYW3, Mito-P, and HB9-GFP homozygous animals, respectively, was determined by RT qPCR. Retinas were collected at P56 for TYW3, P25 for Mito-P, and P6 for HB9-GFP. Values were compared to those from wild-type littermates for Mito-P and HB9-GFP and to age-matched C57BL/6J controls for TYW3. ΔΔ CT values were calculated against Δ CT values of Gapdh. The difference was calculated as 2ˆ-ΔΔCT. The change in expression compared to control was significant for all three transgenic lines: Slm1 in TYW3 homozygotes (p < 0.0001), Fat4 in Mito-P homozygotes (p < 0.0001), and Cdh6 in HB9-GFP homozygotes (p < 0.009) (HB9-GFP n = 4; Mito-P n = 8; TYW3 n = 4). Significance was calculated by one-way ANOVA and Tukey’s multiple comparison tests. Effect of transgene on endogenous gene expression varies with distance between the transgene and the endogenous gene.