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. 2018 Oct 23;9:1536. doi: 10.3389/fpls.2018.01536

FIGURE 1.

FIGURE 1

Representation of DNA constructs used for RNAi-suppression of BEI, BEIIa, and BEIIb gene expression in rice endosperm (Helliwell et al., 2002). (A) The rice polyubiquitin 2 promoter (rice Pub2 promoter) was used and attR1 and attR2 are recombination recognition sites of the target gene. The ccdB protein selectively inhibits Escherichia coli DNA topoisomerase type II and inhibits the growth of most E. coli strains such as OmniMAXTM 2-T1R, DH5αTM, TOP10. This was used for selection for recombination of the target gene. The pdk intron was used to construct the hairpin loop RNA of the RNAi. The CaMV 35S-driven hygromycin resistance gene (hpt intron) was used for selection of transgenic plants. (B) The DNA fragments for the target BE genes used for the seven different RNAi.