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. 2018 Aug 24;5(10):460–468. doi: 10.15698/mic2018.10.652

Figure 1. FIGURE 1: Localization of TCTP in bloodstream trypanosomes.

Figure 1

(A) Western blot of BSF cells extracted with 0.025% digitonin. Total cellular extract (T), supernatant (S) and pellet (P) fractions were analysed by western blot decorated with antibodies against TCTP, ATOM and EF1α.

(B) Immunofluorescence images of BSF cell expressing N-terminally myc-tagged TCTP2 or C-terminal HA-tagged TCTP2. Myc-TCTP2 was detected with anti-myc antibody (white). The DNA is detected by DAPI (cyan). K, kinetoplast DNA; N = nuclear DNA; Phase, phase contrast microscopy; scale bars: 5 µm.