Figure 5.

Influence of pretreatment with flumazenil or WAY100635 on the anxiolytic effects of linalool. The increase in time spent in open arms (A) and number of entries to open arms (B) from linalool odor exposure were eliminated in mice treated with flumazenil, but not with WAY100635 in linalool odor-induced anxiolytic effects. Pretreatment with flumazenil or WAY100635 without linalool odor exposure did not affect exploratory behaviors. Control, mice exposed to odorless air; Linalool, mice exposed to 200 μL of linalool; VEH; mice administered vehicle (2% Tween80 in saline); Flu, mice administered flumazenil (3 mg/kg i.p.); WAY, mice administered WAY100635 (0.5 mg/kg i.p.); each column represents mean ± SEM; n = 10 for all groups. **P < 0.01, ****P < 0.0001 compared to the Control + VEH group, ^###P < 0.001 compared to the Linalool + Flu, ^$$P < 0.01 compared to the Control + WAY (post hoc Tukey’s multiple comparison test).