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. 2018 Oct 23;9:1542. doi: 10.3389/fpls.2018.01542

FIGURE 2.

FIGURE 2

Active site determinants of monocot class II diterpene synthases. Illustrated is a protein sequence alignment highlighting key active site residues with demonstrated impact on product specificity of known monocot class II diTPSs. ZmCPS3 and ZmCPS4 feature distinct residues in select active site positions defining enzyme product specificity, thus suggesting a (+)-CPP synthase, 8,13-CPP synthase, or other specialized class II diTPS function for ZmCPS3 and ZmCPS4. Residue positions are numbered in reference to ZmAN2. Zm, Zea mays; Os, Oryza sativa; Ta, Triticum aestivum; Hv, Hordeum vulgare; Pv, Panicum virgatum.