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. 2018 Oct 29;38(22):e00242-18. doi: 10.1128/MCB.00242-18

FIG 4.

FIG 4

Local overexpression of VEGF-A induces sympathetic activation in sWAT. (A) Whole-mount IF staining with anti-tyrosine hydroxylase (α-TH) antibody (green) in sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding for 7 days. The nuclei were stained with DAPI (blue). (B) NE levels in sWAT and serum of VEGF Tg mice and their littermate controls after HFD-Dox feeding for 7 days. Results for sWAT were normalized to the total protein levels (n = 5 for control group and n = 6 for VEGF Tg group; Student's t test, *, P < 0.05, N.S., not significant). (C) qPCR analysis of adrenergic receptor genes, namely, Adrb3 and Adra2a, in sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding for 7 days (n = 6 per group; Student's t test, *, P < 0.05; **, P < 0.01). (D) cAMP levels in sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding for 7 days (n = 5 per group; Student's t test, *, P < 0.05). (E) IHC staining with anti-phospho-PKA substrate antibody in the sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding for 7 days (scale bar, 20 μm). (F) Whole-mount IF staining with antiendomucin antibody (red) and anti-tyrosine hydroxylase (α-TH) antibody (green) in sWAT of VEGF Tg mice after HFD-Dox feeding for 0 days, 1 day, 2 days, and 7 days (scale bar, 50 μm). (G) qPCR analysis of Ucp1 genes in sWAT of VEGF Tg mice after HFD-Dox feeding for 0 days, 1 day, 2 days, and 7 days (n = 3 per group; Student's t test, **, P < 0.01; ***, P < 0.001).