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. 2018 Oct 29;38(22):e00242-18. doi: 10.1128/MCB.00242-18

FIG 7.

FIG 7

SR59230A suppresses VEGF-A-induced UCP1 expression and mitochondrial function in sWAT. (A) IHC staining with anti-UCP1 antibody in sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding, with or without SR59230A (SR) treatment, for 7 days (scale bar, 50 μm). (B) qPCR analysis of Ucp1 and mitochondrial biogenetic genes including CoxIV and Nrf1 in sWAT of VEGF Tg mice and their littermate controls after HFD-Dox feeding, with or without SR59230A treatment, for 7 days (n = 5 per group; Student's t test, *, P < 0.05). (C) Oxygen consumption rate (OCR) in sWAT measured by a Seahorse instrument. The sWAT was collected from VEGF Tg mice and their littermate controls after HFD-Dox feeding, with or without SR59230A treatment, for 7 days. The injection time point and final concentration of different compounds used for mitochondrial stress assay are indicated in the panel (n = 5 per group).