FIG 3.

A variety of wrapping events occur in BoHV-1-infected cells. MDBK cells were infected at an MOI of 5 with BoHV-1 and fixed and processed for transmission electron microscopy at 12 hpi. (A and B) Representative sections of the nuclear envelope. In panel B, nuclear budding profiles that contain capsids resembling B capsids (white arrow) and A capsids (white arrow with black outline) or those occurring without a capsid (black arrows) are indicated. Bar = 200 nm. INM, inner nuclear membrane; ONM, outer nuclear membrane. (C) Fully wrapped capsidless L-particles were detected in the cytoplasm. Bar = 100 nm. (D) Multiple extracellular particles included full virions and capsidless L-particles. Bar = 500 nm. (E) Extracellular particles of MDBK cells infected with two strains of BoHV-1 were scored for the presence or absence of a capsid. Wt, wild type.