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Role of each exposed residue. (A) Illustration of the minigenome construct and a list of alanine mutations for the surface-exposed residues in the helix 5-loop motif. Cys164 was also mutated to an alanine (green) as a control. (B) Luciferase activities in the minigenome assays. The mutations of the N protein in pN and controls are labeled. (C) Protein expression levels of N mutants in the minigenome assays as determined by Western blotting.
