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(A) Elution profile of NLPs from an S200 size exclusion column. The light blue line corresponds to wt NLP, the red line corresponds to E169A, the dark green line corresponds to F171A, and the light green line corresponds to L174A. (B) TEM images, class averages, and selected particles of the E169A, F171A, and L174A. Class averages were prepared with EMAN2, and particles were selected to show that the mutants still assemble in the same way as the wt rings (10 subunits in each assembly).
