FIG 5.

Verification of compensatory mutations. Luciferase activities were measured in minigenome assays. The horizontal axis is labeled as follows: controls, P169a (pN-E169A, pP, and pL), P169b (pN-E169A, pP, and pL-I753M), P169c (pN-M166I/E169A/R179H, pP, an pL-I753M), P171a (pN-F171A, pP, and pL), P171b (pN-F171A, pP, an pL-R998Q), P171c (pN-L110V/F171A, pP, and pL-R998A), P174a (pN-L174A, pP, and pL), P174b (pN-L174A, pP, and pL-R728K), P174c (pN-L174V, pP-S264Y, and pL-R728K), and P174d (pN-L174A, pP-S264Y, and pL-R728K). Mutations introduced in the plasmids that express the corresponding proteins or the wt plasmids are indicated in parentheses.