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Growth curves of mutant viruses. Compensatory mutations identified in Ni, Nii, and Niii were introduced in the genome of the parent VSV (mcP VSV), and the mutant viruses were rescued (mcP VSV-Ni, mcP VSV-Nii, and mcP VSV-Niii; Table 1). Confluent BHK-21 cells were infected by each marked virus with an MOI of 0.01. Samples were collected from each infection at the given time points, and the virus titers were determined by plaque assays.
