FIG 4.

Replication and protein expression of rVic M2-Baso and rVic M2-FLAG on hNECs. High-MOI infections were performed with the indicated viruses, and 24 hpi virus titers in the apical (A) and basolateral (B) chambers were determined. Data are pooled from three independent replicates. n = 3 wells in each replicate. *, P < 0.01 (one-way ANOVA). (C) Infected cell supernatants and cells were lysed and analyzed by Western blotting with antibodies against HA2, NP, and M1. (D and G) HA2 quantification for cells (D) and apical supernatant (G). (E and H) NP quantification for cells (E) and apical supernatant (H). (F and I) M1 quantification for cells (F) and apical supernatant (I). Data are pooled from two or three independent replicates. n = 3 wells in each replicate. *, P < 0.01 (one-way ANOVA).