FIG 6.

Induction of mitophagy in human primary neurons treated with HIV gp120 and Tat is associated with increased expression and translocation of Parkin to damaged mitochondria. (A) Healthy tubular network of mitochondria (green) and diffuse cytoplasmic expression of Parkin (red). (B to D) Confocal laser microscopy showing translocation of Parkin (red) to the damaged fragmented mitochondria stained with TOM20 (green). In the enlarged images of boxed areas, solid white arrows indicate mitochondrial tubular network and dashed white arrows indicate colocalization of Parkin (red) with TOM20-stained (green) fragmented mitochondria (yellow). Scale bars, 10 μm.