FIG 2.

Production of WNV, DENV2, and ZIKV pseudoviruses. (A) Cytofluorimetry profiles of Vero cells infected with pseudoviruses produced with the different packaging constructs shown in Fig. 1. The profiles are representative of several experiments. (B) Quantification of cytofluorimetry data shown in panel A (n = 12, 9, 15, and 11 for the WNV, DENV2, DENV2 T101G mutant, and ZIKV packaging constructs, respectively). (C) A nonreducing Western blot (upper panels) of cell extracts and pellets of ultracentrifuged supernatants of HEK293T cells cotransfected with the WNV-rep and the indicated packaging constructs is shown. Protein E was detected with the flavivirus-specific mAb 4G2. Actin was used as a loading control. The bottom panel shows the results of agarose gel analysis of RT-PCR products of the same supernatants. DNA stained with ethidium bromide.