Extended Data Figure 4. Increased senescence-associated gene expression is observed in astrocytes and microglia isolated from PS19 mice.

a–e, Gating strategy (a) for FACS isolation of living astrocytes (b), microglia (c), oligodendrocytes (d), and neuron-enriched Cd56⁺ cells (e) from cortices from 6-month-old WT and PS19 mice. b, Astrocyte (Cd11b^–,Cd45^–,O1^–,GLAST⁺,Cd56^–) fraction (left) and RT-qPCR analysis (right). c, Microglia (Cd11b⁺,Cd45⁺,O1,GLAST⁺,Cd56^–) fraction (left) and RT-qPCR analysis (right). d, Oligodendrocyte (Cd11b^–,Cd45^–,O1⁺,GLAST^–,Cd56^–) fraction (left) and RT-qPCR analysis (right). e, Neuron-enriched Cd56⁺ (Cd11b^–,Cd45^–,O1^–,GLAST^–,Cd56⁺) fraction (left) and RT-qPCR analysis (right). p21 is also known as Cdkn1a; Pai1 is also known as Serpine1. Individual numbers of independent animal cell population isolations are indicated in the parentheses above p16^Ink4a columns (2 independent experiments). Data are mean ± s.e.m. *P < 0.05; **P < 0.01 (unpaired two-sided t-tests with Welch’s correction). Exact P values can be found in the accompanying source data file.