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. 2018 Oct 16;2018:1915828. doi: 10.1155/2018/1915828

Figure 5.

Figure 5

IBC-induced PC-3 cell apoptosis is dependent on intracellular ROS generation. (a) Time course of IBC-induced ROS production. As indicated, cells were treated for different lengths of time with 45 μM IBC and then stained with 10 μM DCFH-DA. The fluorescence intensity was measured by flow cytometry. (b) Intracellular IBC-induced ROS generation was measured by flow cytometry. (c-d) Representative images of ROS production in cells treated with IBC in the presence or absence of NAC or BOS. PC-3 cells were preincubated with NAC or BSO for 2 h before incubating with 45 μM IBC for 6 h. Intracellular ROS production was measured by flow cytometry. (e) Representative images of apoptotic cells identified by staining with annexin V-FITC/PI. Cells were treated with 45 μM IBC in the presence or absence of NAC or BSO for 24 h. Percentage of apoptotic cells was determined by annexin V/PI staining and flow cytometry. (f) Quantitative analysis of the rates of apoptotic PC-3 cells after IBC treatment. Data from three independent experiments are presented as mean ± SD. ∗p < 0.05 and ∗∗p < 0.01.