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. 2018 Oct 16;2018:5892957. doi: 10.1155/2018/5892957

Figure 4.

Figure 4

Inhibitory effect of cordycepin on RANKL-induced osteoclast differentiation in RAW264.7 cells. Cells were exposed to 25 ng/ml RANKL for 72 h in the absence or presence of cordycepin. (a) Multinucleated osteoclasts were stained by using a commercially available TRAP assay kit. Osteoclasts were observed under light microscopy (magnification: 100-fold). (b) TRAP-positive multinucleated osteoclasts occupying the area were measured by image J. Cells were counterstained with hematoxylin solution. (c) Differentiated osteoclasts were fixed and stained with DAPI (nuclei) and rhodamine phalloidin (F-actin). Original magnification: 100-fold. (d) Cells were treated with 25 ng/ml RANKL in the absence or presence of the indicated concentration of cordycepin for 72 h on calcium phosphate-coated plates. Pits were observed under light microscopy (magnification: 100-fold). (e) The graph represents the resorption pit area. Data represents the means ± SEM of three independent experiments. ∗P < 0.05 versus positive control.