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. 2018 Oct 29;18:537. doi: 10.1186/s12879-018-3446-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Determination of optimal spiking level for internal quality control. The sensitivity of the metagenomic analysis workflow for MS2 bacteriophage (Internal Quality Control, IQC) detection was evaluated with a MS2 ten-fold serial dilutions in a nasopharyngeal swab tested negative with multiplex viral PCR. Relative abundance of MS2 bacteriophage and viral families are represented depending on the MS2 spiked-in concentration. IQCT1 corresponds to MS2 molecular detection with commercial real-time PCR assay after amplification step. IQCT2 corresponds to control by sequencing metrics (number of MS2 reads normalized with RPKM ratio and MS2 genome coverage)