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. 2018 Oct 29;18:537. doi: 10.1186/s12879-018-3446-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Internal quality control detection after metagenomic analysis of the respiratory samples selected. Distribution of normalized read counts (RPKM) for MS2 bacteriophage (internal quality control, IQC) depending on the proportion of viral reads for the 37 respiratory samples selected. MS2 RPKM was determined after subtracting of NTC MS2 RPKM. IQC was not detected for 4/37 samples (represented in red); among them 3 samples were tested positive with viral real-time PCR