Fig. 3.

The expression of miR393 is induced by diverse stresses in rice. a The expression of miR393a and miR390 in response to ABA and stresses. The RNA levels were detected by qRT-PCR in 2-week-old seedlings exposed to ABA (1.0 μM) and different stresses for 12 h, including drought (25% PEG), pathogen (Magnaporthegrisea), Cu²⁺ (200 μM), UV (100 μmol m^− 2 s^− 1 ultraviolet), NaCl (200 mM). 2-week-old seedlings without stress treatment served as control. *, Significant difference at P < 0.05 compared with No treatment by Student’s t-test (n = 3; means ± SDs). b Time course analysis of miR393a levels in response to ABA (1.0 μM), pathogen (Magnaporthegrisea), and Cu²⁺ (200 μM) by qRT-PCR. *, Significant difference at P < 0.05 compared with No treatment by Student’s t-test (n = 3; means ± SDs). c GUS staining analysis of spatial expression pattern of miR393a under ABA treatment for 12 h in the transgenic plant MIR393a::GUS. The 2-week-old primary roots were stained before and after treatment respectively. Bar = 2 cm. d Time course analysis of miR393a, OsTIR, miR390, and OsARF3 levels in response to ABA. 2-week-old seedlings were incubated into N₆ liquid medium containing 1 μM ABA. Expression was analyzed by qRT-PCR. *, Significant difference at P < 0.05 compared with No treatment by Student’s t-test (n = 3; means ± SDs)