Table 1.
Primer and probe sequences for Treponema species-specific fourplex qPCR and conventional PCR
| Species | Forward primer (sense) | Probe (sense) | Reverse Primer (antisense) | Size (bp) |
|---|---|---|---|---|
| T. denticola a | GGAAACTTAGGAATTCGATATGTAG | AGCATACAGCGATTATAACAAAGCCCTCGA | CCTTCTTTAGTTTCTTTGTGAGG | 113 |
| T. medium a | AAAGCGCTACGAATCCTAAG | TGCACCCTTGTTTACTACTGCACAGCC | ATCATTACCCGTCCACAAAG | 119 |
| T. phagedenis a | CCCGCAGGAAGGTATAATC | AATCCGCCTACGACTGCGATACCA | CACAGCTGTTGTGGTATTAAG | 90 |
| T. pedis a | ACACCGATTGTACTGAATGA | ACTACACGTGGAGTACCGAATGCT | CCACGAGCTTTCTACAGATT | 118 |
| T. denticola b | AGGAATGGCCTTTGAACCCGCA | CCGATGAACCCGTATCTTCACCGA | 458 | |
| T. medium b | GGAACAGGCAGCCGCATTGGAT | CCGCCCATGTGAGGCTTGTGAT | 515 | |
| T. phagedenis b | TCCGCCTACGACTGCGATACCA | CGGAACTGTCACAACTGGCGGA | 785 | |
| T. pedis b | TGGATGTTACGGAAGAGACACCGA | TGCCCCACTCTTACAAGTTCATCCCA | 295 |
aPrimer and probe sequences for species of Treponema for qPCR.
bConventional PCR primer sequences for Treponema species.