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. Author manuscript; available in PMC: 2018 Oct 30.
Published in final edited form as: J Am Chem Soc. 2018 Aug 16;140(34):10639–10643. doi: 10.1021/jacs.8b04638

Figure 1. DNA-corralled nanodisc reconstitution.

Figure 1.

(a) Addition of detergent and lipids (POPC/POPG plus 10% cholesterol) to small nanodisc-decorated barrels (90 nm outer diameter) followed by dialysis results in the reconstitution of DCND. Each DNA barrel is decorated with ssDNA overhang as handles to hybridize with ssDNA chemically conjugated small nanodisc. (b) Negative-stain TEM images of DNA-origami barrel, with 70 nm inner diameter, before (top left) and after (top right) assembly with small nanodiscs. TEM images after dialysis to remove detergent, forming integrated large sized nanodiscs (bottom right) and after ultracentrifugation (UC) to remove free lipid vesicles (bottom left). Scale bar, 100 nm (top insets with 50 nm scale bar). Each step is imaged at lower magnification in Figures S1-S5.