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. Author manuscript; available in PMC: 2018 Oct 30.
Published in final edited form as: Cell Rep. 2018 Sep 11;24(11):2932–2945.e4. doi: 10.1016/j.celrep.2018.08.037

Figure 4. Mitochondrial Stress Promotes Mitochondrial SPHK-1 Accumulation.

Figure 4.

(A) Representative intestinal images of animals co-expressing SPHK-1::GFP and mito-marker TOMM-20::mCherry following 24-hr paraquat treatment. Scale barrepresents 10 μm. Asterisk indicates intestinal nucleus.

(B) Representative images of intestinal SPHK-1::GFP in the absence or presence of paraquat. The yellow asterisk marks the reticulated region within the intestinal cells used to quantify mitochondrial SPHK-1::GFP abundance. The white asterisk marks the region in between mitochondria used to quantify cytosolic SPHK1::GFP abundance (left). Average GFP fluorescence intensity of mitochondrial or cytoplasmic SPHK-1::GFP is quantified (center). The ratio of mitochondrial-tocytosolic SPHK-1::GFP fluorescence is quantified (right). Scale bar indicates 2 mm.

(C) Representative images and quantification of TOMM-20::mCherry in intestinal cells in the absence or presence of paraquat. See (B) for details. Scale barindicates 2 μm.

The sample sizes (n) and ±SEMs are listed in Table S1. Error bars indicate ± SEMs. Student’s t test; p < 0.001