Figure 1. PDE5 Expression and Activity is Increased in SV RV Myocardium.

(A) PDE5 specific enzymatic activity in NF and SV. Bar equals mean±SEM, each point represents individual patient values, n=7 NF and n=13 SV RV samples; asterisk (*) denotes significant difference between groups, unpaired t-test, p<0.05. (B) Total PDE enzymatic activity in NF and SV. Bar equals mean±SEM, each point represents individual patient values, n=7 NF and n=13 SV RV samples. (C) Quantification of PDE5 protein relative to GAPDH protein in NF and SV. Quantitative data were log₂ transformed, bar equals log₂(mean±SEM), each point represents individual patient values, n=9 NF and n=8 SV RV samples; asterisk (*) denotes significant difference between groups, unpaired t-test, p<0.001. A representative blot of PDE5 and GAPDH protein in NF and SV RV is shown on the right.