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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Circ Heart Fail. 2018 Sep;11(9):e004571. doi: 10.1161/CIRCHEARTFAILURE.117.004571

Figure 5. SV Serum Induces Significant Transcriptional Changes and Dysregulated Cardiotoxicity Pathways in vitro.

Figure 5.

(A) Volcano plot representation of all the 20,398 transcripts detected by RNA-Seq depicted as the log2 fold-changes in expression (x-axis) and the log odds of a gene being differentially expressed (y-axis), highlighting the 1,135 transcripts that are significantly differentially expressed between NF versus SV-serum treated NRVMs (above dotted line). ANOVA, -log10(p-value)>1.3 or p<0.05). (B) Heat map of the 1,135 significantly differentially expressed transcripts (raw Fragments Per Kilobase of transcript per Million mapped reads, FPKM values). Unsupervised hierarchical clustering separated NF (n=3) and SV (n=3) serum-treated NRVM samples. (C) Significantly dysregulated canonical pathways in NF versus SV serum-treated NRVMs identified with IPA using the 1,135 transcripts that changed significantly in all 3 SV-treated cell groups. Fisher’s exact test, -log10(p-value)>2.9 or p<0.001. (D) Significantly dysregulated cardiotoxicity functions in NF versus SV serum-treated NRVMs identified with IPA using the 1,135 transcripts that changed significantly in all 3 SV-treated cell groups. Fisher’s exact test, -log10(p-value)>1.3 or p<0.05. (E) Categorization of Gene Ontology (GO) annotations for biological processes in NF versus SV serum-treated NRVMs identified with PANTHER using the 1,135 transcripts that changed significantly in all 3 SV-treated cell groups.