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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Circ Res. 2018 Sep 28;123(8):953–963. doi: 10.1161/CIRCRESAHA.118.313369

Figure 3. Transcriptional and translational modifications of RyR2 in R176Q/+ mice mediated by CRISPR/Cas9 genome editing.

Figure 3.

A, Diagram showing where PCR primers bind to sequences in exons 3-6 upstream of mutation R176Q in exon 8 (green) and primers targeting exons 11-13 downstream of R176Q (orange). B, Levels of RyR2 mRNA in cardiac tissue from R176Q/+ mice or WT littermates, treated with BbsI-SaCas9 or gRNA-SaCas9. C, Western blot analysis of RyR2 and JPH2 protein levels in heart tissue from R176Q/+ mice or WT littermates. D, Corresponding quantifications of RyR2, and E, JPH2 proteins levels normalized to GAPDH. *p<0.05.