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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Circ Res. 2018 Sep 28;123(8):953–963. doi: 10.1161/CIRCRESAHA.118.313369

Figure 5. Validation of the efficiency and specificity of gene editing of the RyR2 mutation allele in cDNA using deep sequencing.

Figure 5.

A, Sequencing amplicon covering exon 6 through 8. Red line marks the mutation in R176Q allele. Target sequence is marked with the blue bar and PAM sequence is marked using the orange bar. B, Frequency of indels in cDNA at the on-target RyR2 site in WT and R176Q/+ mice (n=4). C, Most common indels in cDNA observed at the target site. Insertions are marked with red and deletions are represented by dash line. Target sequence is highlighted in blue and PAM is marked as above. *** p<0.001.