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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Circ Res. 2018 Sep 28;123(8):953–963. doi: 10.1161/CIRCRESAHA.118.313369

Figure 6. AAV9-mediated normalized intracellular Ca2+ handling in R176Q/+ mice.

Figure 6.

A, Schematic picture showing gRNA-SaCas9 and “reporter” AAV9 co-injected in R176Q/+ mice. The “reporter” virus with cloned targeted sequence was cut by gRNA-SaCas9 but not the control BbsI-SaCas9. The mCherry protein is only expressed in cardiomycytes with SaCas9 expression. B, Representative confocal images of ventricle myocytes from R176Q/+ mice co-injected with Bbsl-SaCas9 (control) + “reporter” and gRNA-SaCas9 (editing) + “reporter”, merged with bright field (BF) and red fluorescence (RFP) signal overlay. Scale bar, 100 μm. C, Bar graph showing percentages of mCherry-positive cardiomyocytes. D-F, Summary data for Ca2+ spark frequency, SR Ca2+ load, and SR Ca2+ transient amplitude. *p<0.05, **p<0.01, ***p<0.001.