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. 2018 Oct 30;17:169. doi: 10.1186/s12934-018-1016-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Sugar concentrations in shake-flasks cultivations of a BL21(DE3), b JM109(DE3) and c HMS174(DE3). Sugar-concentrations over time in the supernatant of shake-flask cultivations on DeLisa minimal media with 9 g/L glucose (white crosses) and 9 g/L lactose (grey diamonds). Three cultivations of a BL21(DE3), b JM109(DE3) and c HMS174(DE3) are compared. As BL21(DE3) was the only Gal⁻ strain, accumulation of galactose (dark grey triangles) was observed when lactose was being consumed. The error on sugar measurements is on average 5%