Table 2.
FHT—expressed as soluble protein | CH3H—expressed as inclusion body | ||||
---|---|---|---|---|---|
µ (h−1) | qs,lac (g/g/h) | Specific product titer (mgP/gX) | µ (h−1) | qs,lac (g/g/h) | Specific product titer (mgP/gX) |
0.07 ± 0.015 | 0.23 ± 0.034 | 27 ± 0.9 | 0.08 ± 0.021 | 0.23 ± 0.038 | 390 ± 31 |
0.04 ± 0.014 | 0.12 ± 0.004 | 17 ± 1.2 | 0.04 ± 0.012 | 0.13 ± 0.005 | 240 ± 16 |
0.02 ± 0.001 | 0.055 ± 0.004 | 11 ± 0.3 | 0.02 ± 0.009 | 0.074 ± 0.012 | 15 ± 2 |
0.01 ± 0.004 | 0.029 ± 0.006 | nd |
Analysis of productivity for FHT (expressed as soluble protein) and CH3H (expressed as inclusion body) in dependence on the specific lactose uptake rate in HMS174(DE3) cultivations. Standard deviations were evaluated from triple measurements and calculated by error propagation