Fig. 1.

Apigenin and curcumin induce cell cycle blockage to inhibit melanoma cell growth. a-f A375, A2058, and RPMI-7951 melanoma cells were treated with apigenin or curcumin at various concentrations for 24 h and cell viability was detected by MTT assays. g-i indicated melanoma cells were treated with DMSO as control, curcumin (CCM, 25 μM), or apigenin (APG, 30 μM) for 24 h and cell cycle distribution was analyzed by flow cytometry. The column charts on the right show the quantitation of cells distributed at each stage in percentage. j-k primary melanocytes were treated with curcumin and apigenin at indicated concentrations for 24 h and cell viability was measured by MTT assays. Data were presented as the mean ± S.D. from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001