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. 2018 Oct 23;9:2434. doi: 10.3389/fimmu.2018.02434

Figure 1.

Figure 1

Inhibition of TLR4 signaling by TAK242 prevents skin fibrosis. C57/BL6 mice received daily s.c. injections of PBS or bleomycin alone, or together with TAK242 (10 or 30 mg/kg) or vehicle. Mice were sacrificed at day 22 and skin was harvested for analysis. (A) Masson's Trichome stain. (a) vehicle, (b) bleomycin for 2 weeks (5 days a week), (c) TAK242, and (d) low dose TAK242 (10 mg/kg) and bleomycin, and (e) high dose of TAK242 and bleomycin (30 mg/kg) injected concurrently for 2 weeks (5 day/week). Representative images. Bar = 25 μm. (B) Dermal thickness (means ± s.d. of five determinations/hpf from five mice/group). One-way analysis of variance followed by Sidak's multiple comparison test. *p < 0.05. (C) Real-time quantitative PCR. Results, normalized with GAPDH, are means ± s.d. of triplicate determinations from four mice/group; One-way analysis of variance followed by Sidak's multiple comparison test. *p < 0.05. (D) Immunofluorescence using antibodies to α-smooth muscle actin (αSMA, green) and DAPI (blue). Treatment groups as indicated inset. bleomycin alone for 2 weeks, (c) bleomycin plus TAK242 started concurrently. Representative images. Bar = 50 μm. Relative fluorescence intensities (means from four randomly selected from thee mice/group).