Figure 6.

Inhibition of TLR4 signaling by TAK242 abrogated activated profibrotic fibroblast phenotype. Normal skin fibroblasts were incubated in medium with Fn^EDA (10 μg/ml) for 72 h in absence or presence of TAK242 (3 uM). (A) mRNA levels were determined by real-time qPCR. Results, normalized with GAPDH, are the means ± SD of triplicate determinations from two independent experiments (P < 0.01, one-way ANOVA followed by Sidak's multiple comparison test). (B) Immunofluorescence using antibodies to type I collagen (red), αSMA (green), and Phalloidin (red). Representative images. Scale bar, 50 μm. Relative fluorescence intensities (inset, means from four randomly selected field/well). *p < 0.05.