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. Author manuscript; available in PMC: 2018 Oct 30.
Published in final edited form as: Sci Transl Med. 2015 Nov 4;7(312):312ra176. doi: 10.1126/scitranslmed.aab1803

Fig. 2: FACT and MYCN act in a transcriptional positive feedback loop.

Fig. 2:

(A) Mean mRNA expression for SPT16, SSRP1, or MYCN in BE(2)C and KELLY cells treated with control, MYCN-1 or MYCN-2 siRNA. Data displayed were obtained 48 hours after transfection and were normalized to mRNA expression in a control siRNA sample. β2-Microglobulin (β2M) was used as a reference gene. *, p<0.05; **, p<0.01; ***, p<0.001. See table S7 for individual p-values. (B) Western blots for SPT16, SSRP1, and MYCN protein expression in BE(2)C cells and KELLY cells treated with control, MYCN-1, or MYCN-2 siRNA. Data displayed were obtained 48 hours after transfection. GAPDH was used as a loading control. (C) Mean mRNA expression for SPT16, SSRP1, or MYCN in BE(2)C and KELLY cells treated with control or combined SSRP1 and SPT16 siRNA. Data displayed were obtained 72 hours after transfection and normalized to mRNA expression in a control siRNA sample. β2M was used as a reference gene. ***, p<0.001. See table S7 for individual p-values. (D) Western blots for SPT16, SSRP1, and MYCN protein expression in BE(2)C cells and KELLY cells treated with control or combined SSRP1 and SPT16 siRNA. Data displayed were obtained 72 hours after transfection. GAPDH was used as a loading control.