Fig. 2.

Localization of wild-type and mutant ADRB2 in U-2 OS cells. Confocal images in the Z and Y planes of wild-type U-2 OS ADRB2 (A–E) and ADRB2(W158A) (F–J) in U-2 OS cells harboring the β-gal EFC components. The distribution of ADRB2(W158A) and co-localization with the ER-specific dye verify retention of mutant receptors within the ER. Boxes indicate regions of interest selected for Y-axis projections inset. ADRB2 receptor (green) was visualized using an anti-PK-tag primary and Alexa-488-conjugated secondary antibody. The ER is stained red. Nuclei stained with DAPI are given in blue. Scale bar size is 10 μm. DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; EFC, enzyme-fragment complementation.