Moderate alcohol intake induces beige adipogenesis in iWAT. C57BL6 mice were supplemented with 0 or 8% (w/v) alcohol for 1 mo. A) Body weight. B) Food intake. C) Total caloric intake. D) Adipose tissue weight. E) Representative images of hematoxylin- and eosin-stained iWAT tissue sections. F) Distribution of iWAT lipid droplet diameter. G) Average iWAT adipocyte diameter. H) Representative images of immunohistochemistry of iWAT sections using anti-UCP1 Ab. I) mRNA levels of Prdm16 (PR domain-containing 16), Ppargc1a (peroxisome proliferator-activated receptor-γ coactivator 1 α), Pparg (peroxisome proliferator-activated receptor-γ), Ucp1 (uncoupling protein 1), Elovl3 (elongation of very long chain fatty acids protein 3), Cox7a1 (cytochrome c oxidase subunit VIIa 1), Cidea (cell death-inducing DNA fragmentation factor α-like effector A), and Zfp423 (zinc-finger protein 423) in iWAT. eWAT, epidydimal WAT. *P < 0.05 (n = 8, mean ± sem).