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. 2017 Jun 8;31(10):4256–4264. doi: 10.1096/fj.201601282R

Figure 2.

Figure 2.

Confocal imaging showed colocalization of AQP1 and band 3 on the surface of erythrocyte vesicles. A) Human RBC ghosts were resealed to form IOVs or ROVs. These vesicles were stained with the lipid-bound fluorophore Di-8-ANEPPS for visualization. B) Band 3 on the surface of IOVs was labeled with BRIC170-Alexa Fluor 488 (green fluorescence) and anti-AQP1-Alexa Fluor 568 (red fluorescence). Immunofluorescence-labeled IOVs from non-Mi.III (WT) and Mi.III RBC-IOVs showed substantial expressions of band 3 and AQP1. Various shades of orange and yellow fluorescence indicate different degrees of colocalization between band 3 and AQP1 on the surface of IOVs. Scale bars, 20 μm.