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Changes in Cl− flux did not affect AQP1-AE1 interaction in erythrocyte IOVs. In this set of experiments, IOVs singly labeled with BRIC170-Alexa Fluor 488 conjugate, or colabeled with anti-AQP1-Alexa Fluor 568, were suspended in half-Cl− HBSS for FLIM measurements. Despite the reduced Cl−, fluorescence lifetimes of FRET donor Eg decreased significantly in the presence of the acceptor Qr. Each group was compiled from 6 to 12 sample measurements (means ± sem). **P < 0.001.
